Protein A anticorps (Biotin)

N° du produit ABIN7565826

Cet anticorps anti-Protein A est un anticorps Chèvre Polyclonal détectant Protein A dans ELISA, WB, IHC et DB. Adapté pour Staphylococcus aureus.

Aperçu rapide pour Protein A anticorps (Biotin) (ABIN7565826)

Antigène: Protein A

Reactivité: Staphylococcus aureus

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Protein A est conjugé à/à la Biotin

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Dot Blot (DB)

Détail du produit anti-Protein A anticorps (Biotin)

Fonction: Protein A Antibody Biotin Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Protein A [Staphylococcus aureus].

Attributs du produit: Synonyms: goat anti-Protein A Antibody, Biotin Conjugated goat anti-Protein A Antibody, Protein A Biotin, Protein A BAC Antibody

Purification: Anti-Protein A is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Protein A [Staphylococcus aureus]

Immunogen Type: Native Protein

Information d'application

Indications d'application: Application Note: Anti-Protein A Biotin Conjugated has been tested by dot blot and western blot. This product is suitable to be assayed against 1.0 μg of Protein A in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product. Immunohistochemistry Dilution: 1:500-1:2,500 Western Blot Dilution: 1:1,000-1:5,000 ELISA Dilution: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détails sur Protein A

Antigène: Protein A

Sujet: Background: Biotin is widely used throughout the biotechnology industry to conjugate proteins for biochemical assays. Biotin's small size typically does not affect the biological activity of protein upon biotinylation. Biotinylated proteins of interest can be enriched from a sample due to highly stable interactions. Biotin conjugated anti-Protein A antibodies are used as an amplifying reagent in immunohistochemistry, microarray assays, ELISAs, blots, and other applications.